genechip gene expression microarray platforms  (Thermo Fisher)

Journal: Epigenetics

Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

doi: 10.1080/15592294.2024.2337087

Figure Lengend Snippet: Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.

Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the Agilent SurePrint G3 Human Gene Expression 8 × 60K microarray platform.

Techniques: Methylation, Isolation, Marker, Generated, Microarray

Journal: Epigenetics

Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

doi: 10.1080/15592294.2024.2337087

Figure Lengend Snippet: Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.

Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the Agilent SurePrint G3 Human Gene Expression 8 × 60K microarray platform.

Techniques: Microarray, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Global Expression of Molecular Transporters in the Human Vaginal Tract: Implications for HIV Chemoprophylaxis

doi: 10.1371/journal.pone.0077340

Figure Lengend Snippet: The box extends from the 25th to 75th percentiles, with the horizontal line in the box representing the median; whiskers represent the lowest and highest datum. ( A ) Normalized probe intensity data from microarray experiments. ( B ) Normalized RT-qPCR measurements, expressed as 1/[Δ(C t )] such that increased values correspond to increased gene expression.

Article Snippet: Gene expression profiles were measured using the Agilent one-color (Cy3 fluorochrome) microarray-based gene expression platform according to manufacturer's instructions.

Techniques: Microarray, Quantitative RT-PCR, Expressing

Journal: PLoS ONE

Article Title: Global Expression of Molecular Transporters in the Human Vaginal Tract: Implications for HIV Chemoprophylaxis

doi: 10.1371/journal.pone.0077340

Figure Lengend Snippet: Correlation of gene expression for the six ARV membrane transporter genes ( ABCB1 , ABCC1 , ABCC2 , ABCC3 , ABCC4 , ABCG2 ) across microarray and qPCR platforms ( R 2 = 0.70, P < 2.2×10 -16 ).

Article Snippet: Gene expression profiles were measured using the Agilent one-color (Cy3 fluorochrome) microarray-based gene expression platform according to manufacturer's instructions.

Techniques: Expressing, Microarray

Journal: OncoTargets and therapy

Article Title: WISP3 is highly expressed in a subset of colorectal carcinomas with a better prognosis

doi: 10.2147/OTT.S97025

Figure Lengend Snippet: Outlier gene analysis from TCGA dataset and three independent CRC microarray cohorts. Notes: Top 20 genes ranked in 90th and 75th outlier genes from TCGA dataset ( A ); No WISP3 positive cases defined by 90th and 75th cutoff was observed in normal colorectal tissues ( B ); WISP3 demonstrated significant outlier expression model in three independent CRC cohorts, Vilar Colorectal 2 ( C ); Vilar Colorectal ( D ); Smith Colorectal ( E ). Abbreviations: COPA, cancer outlier profile analysis; CRC, colorectal cancer; TCGA, The Cancer Genome Atlas; WISP3, WNT1-inducible-signaling pathway protein 3.

Article Snippet: TCGA mRNA expression data were produced on Agilent 244K Custom Gene Expression microarray platform (Agilent Technologies, Santa Clara, CA, USA) and Illumina RNA-Seq platform (Illumina, Inc., San Diego, CA, USA).

Techniques: Microarray, Expressing